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CABRI:DSMZ_MUTZ |
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Accession_number |
ACC 431
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Cell_line_name |
42-MG-BA
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Brief_description |
confirmed as human with IEF of LDH, MDH, NP established from the temporal lobe
brain biopsy from a 63-year-old man with glioblastoma multiforme in 1990 (prior to
treatment)
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Description |
human glioma established from the temporal lobe brain biopsy from a 63-year-old
man with glioblastoma multiforme in 1990 (prior to treatment) confirmed as human with
IEF of LDH, MDH, NP
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Depositor |
Dr. A. Perzelova, Comenius University, Bratislava, Slovakia
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Morphology |
flat epithelial-like polygonal cells growing adherently as monolayer or, if confluent, as
multilayers with processes
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Culture_conditions |
80% mixture of RPMI 1640 + MEM (with Earle's salts) (at 1:1) + 20% FBS split
confluent culture 1:2 to 1:4 every 3-6 days using trypsin/EDTA; seed out at ca. 1 x 106
cells/80 cm 2 ; saturation density at ca. 0.6 x 105 /cm 2 at 37C with 5% CO2 cell
harvest of maximal 0.3-0.5 x 105 /cm 2
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Viruses |
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV -,
HTLV-I/II -, SMRV -
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Properties |
cytokeratin -, desmin -, endothel -, GFAP +, neurofilament -, vimentin +
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Release_conditions |
-
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Hazard |
-
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Storage |
frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 106 cells/ampoule
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Doubling_time |
ca. 40 hours
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Mycoplasma |
contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI,
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Fingerprint |
multiplex PCR of minisatellite markers revealed a unique DNA profile
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Cytogenetics |
human flat-moded hypertetraploid karyotype with 8% polyploidy - 88-95<4n>XX, -Y, -
Y, +4, -5, -5, -7, -8, -9, -9, +11, +11, -13, -13, +14, +14, +15, -16, -17, -17, -18, -18, -
19, -21, -22, +14mar, add(1)(p1?), del(1)(q21.2), del(2)(q13)x2,
dup(2)(p12p2?4)add(2)(p2?4), del(3)(q13.3), add(4)(p11), add(5)(p1?5),
add(6)(q2?)x2, add(7)(q11)x2, add(7)(q3?), del(9)(p21p22)x2, del(11)(p11)x2,
inv(12)(q12q21)x2, der(12)del(p11)t(7;12)(q21;q1?4)x2, add(13)(p11)x2,
add(14)(q32)x2, add(21)(p11)x2
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Bibliographic_reference |
Perzelova et al., Neoplasma, 45: 29 (1998) [PMID: 9604998
] |
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